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tu6 pytk095 addgene  (Addgene inc)


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    Structured Review

    Addgene inc tu6 pytk095 addgene
    Tu6 Pytk095 Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tu6+pytk095+addgene/pYTK095+(Plasmid+%2365202)/pmc08966344__13007_2022_867_MOESM1_ESM-12-352-354
    Average 93 stars, based on 10 article reviews
    tu6 pytk095 addgene - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Control:

    Article Title: Orthogonal control of gene expression in plants using synthetic promoters and CRISPR-based transcription factors
    Article Snippet: YFP under the control of pATF-1 promoter TU1 : EBS: HHR-gRNA-1-HDV:T35S (917 bp) TU2 : pATF-1 : YFP: T35S (1231 bp) TU3E : P35S : dCas9:VP64: T35S (5162 bp) OCS 4-1 (14,627bp) U6 driven constitutive expression of gRNA-1 leading to constitutive expression of Luc under the control of pATF-1 promoter TU1 : U6 :gRNA-1 (1013 bp) TU2 : pATF-1 : Luc: T35S (2150 bp) TU3E : P35S : dCas9:VP64: T35S (5162 bp) OCS 3-5 (16,989bp) Ratiometric circuit where YFP under the control of pATF-1 inducible by ethylene, while RFP and BFP under the control of Patf-3 are constitutively expressed TU1 : EBS: HHR-gRNA-1-HDV:T35S (917 bp) TU2 : pATF-1 : YFP: T35S (1231 bp) TU3: pATF-3 : BFP : T35S (1228 bp) TU4: U6 :gRNA3 (1013 bp) TU5: pATF-3 : RFP : T35S (1214 bp) TU6E : P35S : dCas9:VP64: T35S (5162 bp) 112 113 Table S3: List of Addgene plasmids used in this study 114 Name Description pICH86966 Addgene #48075 Used as the backbone of the shuttle vector; Contains the pVS1 and pMB1 replicons, KanR (bacterial) as well as a plant KanR under pNos. pYTK001 Addgene #65108 Backbone for cloning all the parts listed in Table S1 pYTK002 Addgene #65109 Contains the connector LS; Used for the construction of TU1 pYTK003 Addgene #65110 Contains the connector L1; Used for the construction of TU2 pYTK004 Addgene #65111 Contains the connector L2; Used for the construction of TU3 pYTK005 Addgene #65112 Contains the connector L3; Used for the construction of TU4 pYTK006 Addgene #65113 Contains the connector L4; Used for the construction of TU5 pYTK007 Addgene #65114 Contains the connector L5; Used for the construction of TU6 pYTK067 Addgene #65174 Contains the connector R1; Used for the construction of TU1 pYTK068 Addgene #65175 Contains the connector R2; Used for the construction of TU2 pYTK069 Addgene #65176 Contains the connector R3; Used for the construction of TU3 pYTK070 Addgene #65177 Contains the connector R4; Used for the construction of TU4 pYTK071 Addgene #65178 Contains the connector R5; Used for the construction of TU5 pYTK072 Addgene #65179 Contains the connector RE; Used for the construction of TU6 pYTK095 Addgene #65202 Used as the backbone for the construction of any transcriptional unit.

    Expressing:

    Article Title: Orthogonal control of gene expression in plants using synthetic promoters and CRISPR-based transcription factors
    Article Snippet: YFP under the control of pATF-1 promoter TU1 : EBS: HHR-gRNA-1-HDV:T35S (917 bp) TU2 : pATF-1 : YFP: T35S (1231 bp) TU3E : P35S : dCas9:VP64: T35S (5162 bp) OCS 4-1 (14,627bp) U6 driven constitutive expression of gRNA-1 leading to constitutive expression of Luc under the control of pATF-1 promoter TU1 : U6 :gRNA-1 (1013 bp) TU2 : pATF-1 : Luc: T35S (2150 bp) TU3E : P35S : dCas9:VP64: T35S (5162 bp) OCS 3-5 (16,989bp) Ratiometric circuit where YFP under the control of pATF-1 inducible by ethylene, while RFP and BFP under the control of Patf-3 are constitutively expressed TU1 : EBS: HHR-gRNA-1-HDV:T35S (917 bp) TU2 : pATF-1 : YFP: T35S (1231 bp) TU3: pATF-3 : BFP : T35S (1228 bp) TU4: U6 :gRNA3 (1013 bp) TU5: pATF-3 : RFP : T35S (1214 bp) TU6E : P35S : dCas9:VP64: T35S (5162 bp) 112 113 Table S3: List of Addgene plasmids used in this study 114 Name Description pICH86966 Addgene #48075 Used as the backbone of the shuttle vector; Contains the pVS1 and pMB1 replicons, KanR (bacterial) as well as a plant KanR under pNos. pYTK001 Addgene #65108 Backbone for cloning all the parts listed in Table S1 pYTK002 Addgene #65109 Contains the connector LS; Used for the construction of TU1 pYTK003 Addgene #65110 Contains the connector L1; Used for the construction of TU2 pYTK004 Addgene #65111 Contains the connector L2; Used for the construction of TU3 pYTK005 Addgene #65112 Contains the connector L3; Used for the construction of TU4 pYTK006 Addgene #65113 Contains the connector L4; Used for the construction of TU5 pYTK007 Addgene #65114 Contains the connector L5; Used for the construction of TU6 pYTK067 Addgene #65174 Contains the connector R1; Used for the construction of TU1 pYTK068 Addgene #65175 Contains the connector R2; Used for the construction of TU2 pYTK069 Addgene #65176 Contains the connector R3; Used for the construction of TU3 pYTK070 Addgene #65177 Contains the connector R4; Used for the construction of TU4 pYTK071 Addgene #65178 Contains the connector R5; Used for the construction of TU5 pYTK072 Addgene #65179 Contains the connector RE; Used for the construction of TU6 pYTK095 Addgene #65202 Used as the backbone for the construction of any transcriptional unit.

    Plasmid Preparation:

    Article Title: Orthogonal control of gene expression in plants using synthetic promoters and CRISPR-based transcription factors
    Article Snippet: YFP under the control of pATF-1 promoter TU1 : EBS: HHR-gRNA-1-HDV:T35S (917 bp) TU2 : pATF-1 : YFP: T35S (1231 bp) TU3E : P35S : dCas9:VP64: T35S (5162 bp) OCS 4-1 (14,627bp) U6 driven constitutive expression of gRNA-1 leading to constitutive expression of Luc under the control of pATF-1 promoter TU1 : U6 :gRNA-1 (1013 bp) TU2 : pATF-1 : Luc: T35S (2150 bp) TU3E : P35S : dCas9:VP64: T35S (5162 bp) OCS 3-5 (16,989bp) Ratiometric circuit where YFP under the control of pATF-1 inducible by ethylene, while RFP and BFP under the control of Patf-3 are constitutively expressed TU1 : EBS: HHR-gRNA-1-HDV:T35S (917 bp) TU2 : pATF-1 : YFP: T35S (1231 bp) TU3: pATF-3 : BFP : T35S (1228 bp) TU4: U6 :gRNA3 (1013 bp) TU5: pATF-3 : RFP : T35S (1214 bp) TU6E : P35S : dCas9:VP64: T35S (5162 bp) 112 113 Table S3: List of Addgene plasmids used in this study 114 Name Description pICH86966 Addgene #48075 Used as the backbone of the shuttle vector; Contains the pVS1 and pMB1 replicons, KanR (bacterial) as well as a plant KanR under pNos. pYTK001 Addgene #65108 Backbone for cloning all the parts listed in Table S1 pYTK002 Addgene #65109 Contains the connector LS; Used for the construction of TU1 pYTK003 Addgene #65110 Contains the connector L1; Used for the construction of TU2 pYTK004 Addgene #65111 Contains the connector L2; Used for the construction of TU3 pYTK005 Addgene #65112 Contains the connector L3; Used for the construction of TU4 pYTK006 Addgene #65113 Contains the connector L4; Used for the construction of TU5 pYTK007 Addgene #65114 Contains the connector L5; Used for the construction of TU6 pYTK067 Addgene #65174 Contains the connector R1; Used for the construction of TU1 pYTK068 Addgene #65175 Contains the connector R2; Used for the construction of TU2 pYTK069 Addgene #65176 Contains the connector R3; Used for the construction of TU3 pYTK070 Addgene #65177 Contains the connector R4; Used for the construction of TU4 pYTK071 Addgene #65178 Contains the connector R5; Used for the construction of TU5 pYTK072 Addgene #65179 Contains the connector RE; Used for the construction of TU6 pYTK095 Addgene #65202 Used as the backbone for the construction of any transcriptional unit.

    Cloning:

    Article Title: Orthogonal control of gene expression in plants using synthetic promoters and CRISPR-based transcription factors
    Article Snippet: YFP under the control of pATF-1 promoter TU1 : EBS: HHR-gRNA-1-HDV:T35S (917 bp) TU2 : pATF-1 : YFP: T35S (1231 bp) TU3E : P35S : dCas9:VP64: T35S (5162 bp) OCS 4-1 (14,627bp) U6 driven constitutive expression of gRNA-1 leading to constitutive expression of Luc under the control of pATF-1 promoter TU1 : U6 :gRNA-1 (1013 bp) TU2 : pATF-1 : Luc: T35S (2150 bp) TU3E : P35S : dCas9:VP64: T35S (5162 bp) OCS 3-5 (16,989bp) Ratiometric circuit where YFP under the control of pATF-1 inducible by ethylene, while RFP and BFP under the control of Patf-3 are constitutively expressed TU1 : EBS: HHR-gRNA-1-HDV:T35S (917 bp) TU2 : pATF-1 : YFP: T35S (1231 bp) TU3: pATF-3 : BFP : T35S (1228 bp) TU4: U6 :gRNA3 (1013 bp) TU5: pATF-3 : RFP : T35S (1214 bp) TU6E : P35S : dCas9:VP64: T35S (5162 bp) 112 113 Table S3: List of Addgene plasmids used in this study 114 Name Description pICH86966 Addgene #48075 Used as the backbone of the shuttle vector; Contains the pVS1 and pMB1 replicons, KanR (bacterial) as well as a plant KanR under pNos. pYTK001 Addgene #65108 Backbone for cloning all the parts listed in Table S1 pYTK002 Addgene #65109 Contains the connector LS; Used for the construction of TU1 pYTK003 Addgene #65110 Contains the connector L1; Used for the construction of TU2 pYTK004 Addgene #65111 Contains the connector L2; Used for the construction of TU3 pYTK005 Addgene #65112 Contains the connector L3; Used for the construction of TU4 pYTK006 Addgene #65113 Contains the connector L4; Used for the construction of TU5 pYTK007 Addgene #65114 Contains the connector L5; Used for the construction of TU6 pYTK067 Addgene #65174 Contains the connector R1; Used for the construction of TU1 pYTK068 Addgene #65175 Contains the connector R2; Used for the construction of TU2 pYTK069 Addgene #65176 Contains the connector R3; Used for the construction of TU3 pYTK070 Addgene #65177 Contains the connector R4; Used for the construction of TU4 pYTK071 Addgene #65178 Contains the connector R5; Used for the construction of TU5 pYTK072 Addgene #65179 Contains the connector RE; Used for the construction of TU6 pYTK095 Addgene #65202 Used as the backbone for the construction of any transcriptional unit.



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    tu6 pytk095 addgene  (Addgene inc)
    93
    Addgene inc tu6 pytk095 addgene
    Tu6 Pytk095 Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tu6+pytk095+addgene/pYTK095+(Plasmid+%2365202)/pmc08966344__13007_2022_867_MOESM1_ESM-12-352-354
    Average 93 stars, based on 1 article reviews
    tu6 pytk095 addgene - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

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